June 25, 2018
Assay System:
- Fresh whole blood from desired species.
- Human blood is mixed gender. All other species are male.
- Stability determined by measuring percent remaining of test compound at multiple time points during incubation in blood.
Assay Conditions:
| Test Compound Concentration | 1 µM |
| Percent Organic Solvent in Incubation | 0.5% acetonitrile |
| Incubation Time | 0, 15, 30, 60, and 90 minutes |
| Temperature | 37 °C |
| Analysis | LC-MS (LC-MS/MS if necessary) |
| Number of Repeats | 2 |
Deliverable: Half-life of test compound.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.