June 25, 2018
Assay System:
- Phosphate Buffer Saline pH 7.4 (PBS); other aqueous systems available upon request.
- Kinetic aqueous solubility measured by incubating test compound in PBS for 1 h, followed by determination of dissolved concentration against a calibration curve.
Assay Conditions:
| Test Compound Concentration | 100 µM |
| Percent Organic Solvent in Incubation | 1% DMSO |
| Incubation Time | 1 hours |
| pH | 7.4 or as requested |
| Temperature | Ambient |
| Positive Control | Verapamil and Tamoxifen |
| Analysis | UV Absorbance or LC-MS (LC-MS/MS if necessary) |
| Number of Repeats | 2 |
Deliverable: Solubility of test compound (µM).
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.