June 25, 2018
Assay System:
- Blood plasma from desired species.
- Stability determined by measuring percent remaining of test compound at multiple time points during incubation in plasma.
Assay Conditions:
| Test Compound Concentration | 10 µM |
| Percent Organic Solvent in Incubation | 0.5% acetonitrile |
| Incubation Time | 0, 15, 30, 60, and 90 minutes |
| pH | 7.4 |
| Temperature | 37 °C |
| Positive Control | Propantheline and/or Enalapril |
| Analysis | LC-MS (LC-MS/MS if necessary) |
| Number of Repeats | 2 |
Deliverable: Half-life of test compound.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.